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Bioamperometric Systems with Fructose Dehydrogenase From Gluconobacter japonicus for D‐Tagatose Monitoring
Author(s) -
Voitechovič Edita,
Stankevičiūtė Jonita,
Vektarienė Aušra,
Vektaris Gytis,
Jančienė Regina,
Kuisienė Nomeda,
Razumienė Julija,
Meškys Rolandas
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060573
Subject(s) - fructose , chemistry , dehydrogenase , biochemistry , enzyme
D‐Tagatose is a rare natural sugar, which is promising alternative for popular dietary sweeteners. To optimize and make D‐tagatose production more cost‐effective, a portable (bio)sensor would be beneficial for D‐tagatose quantification in bioreactor media during D‐tagatose (bio)synthesis. In this study, a pilot study of D‐tagatose quantification by a bioamperometric system is reported. A key feature of the system is usage of fructose dehydrogenase from Gluconobacter japonicus , widely known as a highly fructose‐specific enzyme, and 2‐arylamino‐1,4‐benzoquinone derivatives, as electron transfer mediators from the enzyme to carbon electrodes. The system has been validated as biosensor for D‐tagatose quantification in the fermentation medium.