Premium
Neuraminidase Based Electro‐Nano Diagnostic Platforms: Development of Model Systems for Cancer Diagnosis
Author(s) -
Tepeli Büyüksünetçi Yudum,
Anik Ülkü
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060563
Subject(s) - detection limit , hela , biosensor , sialic acid , electrode , platinum , neuraminidase , chemistry , electrochemistry , materials science , analytical chemistry (journal) , chromatography , nanotechnology , cell , biochemistry , enzyme , catalysis
An electrochemical biosensor that monitored neuraminidase (NEU3), activity was developed. The analysis platform included a graphene‐platinum hybrid modified gold screen printed electrode as a transducer. The detection protocol was based on observation of NEU3 activity which was used to remove sialic acid from the GD3 ganglioside. Examination of analytical characteristics resulted with two linear ranges of 10 −8 U/mL–10 −1 and 10 −1 U/mL–2.53 U/mL with limit of detection values of 10 −8 U/mL and 10 −1 U/mL, respectively. The selectivity of the developed NEU3 activity based electrochemical biosensor was tested with HeLa, VERO and A549 cell lines.