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Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes
Author(s) -
Lefrançois Pauline,
GirardSahun Fanny,
Badets Vasilica,
Clément Franck,
Arbault Stéphane
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060456
Subject(s) - superoxide , chemistry , redox , microelectrode , aqueous solution , ion , oxygen , reactivity (psychology) , inorganic chemistry , reactive oxygen species , phosphate , electrochemistry , electrode , biochemistry , organic chemistry , enzyme , alternative medicine , pathology , medicine
The reactivity of platinized ultramicroelectrodes (Pt‐black UMEs) towards superoxide anion O 2 .− , an unstable Reactive Oxygen Species (ROS), and its relatives, H 2 O 2 and O 2 , was studied. Voltammetric studies in PBS demonstrate that Pt‐black UMEs provide: i) a well‐resolved reversible redox signature for O 2 .− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H 2 O 2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt‐black surfaces solely yields H 2 O 2 (2 electrons/2 H + ) at physiological pH. Consequently, Pt‐black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico‐chemical investigations.