Amperometric Detection of Creatinine in Clinical Samples Based on Gold Electrode Arrays Fabricated Using Printed Circuit Board Technology | Zendy

Ortiz Mayreli | Zendy; Botero Mary Luz | Zendy; Fragoso Alex | Zendy; O'Sullivan Ciara K. | Zendy

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Amperometric Detection of Creatinine in Clinical Samples Based on Gold Electrode Arrays Fabricated Using Printed Circuit Board Technology

Author(s) -

Ortiz Mayreli,

Botero Mary Luz,

Fragoso Alex,

O'Sullivan Ciara K.

Publication year - 2020

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.202060446

Subject(s) - amperometry , printed circuit board , creatinine , electrode , linear range , materials science , polyaniline , biosensor , biomedical engineering , nafion , chromatography , detection limit , chemistry , electrochemistry , medicine , nanotechnology , computer science , composite material , biochemistry , polymer , polymerization , operating system

Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit technology. A linear range of 1–1000 μM was obtained with standard deviations of less than 5 % (n=3) obtained. Stability studies were carried out and 80 % activity was maintained after one month. The sensors were tested with 142 serum samples from patients with renal dysfunction at different stage of the disease and validated using a commercial colorimetric method.

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