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Amperometric Detection of Creatinine in Clinical Samples Based on Gold Electrode Arrays Fabricated Using Printed Circuit Board Technology
Author(s) -
Ortiz Mayreli,
Botero Mary Luz,
Fragoso Alex,
O'Sullivan Ciara K.
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060446
Subject(s) - amperometry , printed circuit board , creatinine , electrode , linear range , materials science , polyaniline , biosensor , biomedical engineering , nafion , chromatography , detection limit , chemistry , electrochemistry , medicine , nanotechnology , computer science , composite material , biochemistry , polymer , polymerization , operating system
Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit technology. A linear range of 1–1000 μM was obtained with standard deviations of less than 5 % (n=3) obtained. Stability studies were carried out and 80 % activity was maintained after one month. The sensors were tested with 142 serum samples from patients with renal dysfunction at different stage of the disease and validated using a commercial colorimetric method.