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Electrochemically Deposited Porous Graphene−Polypyrrole−Polyphenol Oxidase for Dopamine Biosensor
Author(s) -
Ramu P.,
Vimal S. P.,
Suresh P.,
Saravanakumar U.,
Sethuraman V.,
Anandhavelu S.
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060400
Subject(s) - detection limit , polypyrrole , graphene , biosensor , electrode , materials science , polyphenol oxidase , linear range , dopamine , chemistry , chromatography , nuclear chemistry , nanotechnology , electrochemistry , organic chemistry , enzyme , biology , peroxidase , neuroscience
We report a method for the fabrication of glassy carbon electrode modified porous graphene‐polypyrrole‐polyphenol oxidase (GCE−PG−PPy−PPO) modified electrode for the determination dopamine. The optimization of pH, concentration and detection limit of dopamine was employed by amperomatric technique. The detection limit of dopamine was found to be in a linear range of 2×10 −8 to 4.6×10 −5 M and lower limit detection is 4×10 −9 M. Michealis – Menten constant (K m ) and the activation energy were calculated as 31.32 μM and 37.4−Kj mol −1 , respectively. The developed biosensor was used to quantify the dopamine in human urine sample.