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An Electrochemical ATP Biosensor with Enzymes Entrapped within a PEDOT Film
Author(s) -
Peteu Serban F.,
Russell Skye A.,
Galligan James J.,
Swain Greg M.
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060397
Subject(s) - biosensor , glucose oxidase , pedot:pss , detection limit , analyte , hexokinase , selectivity , electrochemistry , conductive polymer , chemistry , combinatorial chemistry , polymer , materials science , chromatography , nuclear chemistry , analytical chemistry (journal) , nanotechnology , enzyme , organic chemistry , electrode , catalysis , glycolysis
This paper reports on the electrochemical behavior of an ATP biosensor that utilizes glucose oxidase (GOx) and hexokinase (Hex) immobilized within the electroactive polymer, polyethylenedioxythiophene (PEDOT). This biosensor design detects ATP indirectly at 0.85 V vs. Ag/AgCl based on the oxidation current for enzymatically generated H 2 O 2 , and at −0.20 V; a potential at which improved analyte selectivity is achieved. The detection figures of merit at both detection potentials are a response time of 15±1 s, an experimental detection limit of 10.0±0.2 μmol L −1 (S/N=3), and a sensitivity in the range of 100–500 mA M −1 cm −2 .