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Human Serum Albumin−Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA‐200c
Author(s) -
Akbal Vural Oznur,
Yaman Yesim Tugce,
Bolat Gulcin,
Abaci Serdar
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060342
Subject(s) - detection limit , colloidal gold , human serum albumin , biosensor , chemistry , nanoparticle , reproducibility , chromatography , oligonucleotide , hybridization probe , selectivity , adsorption , analytical chemistry (journal) , dna , biophysics , nanotechnology , materials science , biochemistry , biology , organic chemistry , catalysis
Here, human serum albumin conjugated gold nanoparticles (HSA−AuNPs) were synthesized by a simple route to develop an impedimetric sensor for miRNA‐200c detection based on a selective oligo‐hybridization process without any labeling. The synthetic DNA capture probe for miRNA‐200c was decorated onto the HSA−AuNPs modified pencil graphite electrodes. Impedimetric signals were monitored after the hybridization process between the DNA probe and target miRNA‐200c. HSA−AuNPs adsorption time, incubation time of the capture probe and hybridization time‐temperature were optimized. The proposed miRNA‐200c biosensor demonstrated proper sensitivity and selectivity, low detection limit (1.13 fM), good reproducibility and simple direct detection of miRNA‐200c in serum.