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Choline Oxidase Based Composite ZrO 2 @AuNPs with Cu 2 O@MnO 2 Platform for Signal Enhancing the Choline Biosensors
Author(s) -
Ouiram Tik,
Moonla Chocha,
Preechaworapun Anchana,
Muangpil Sairoong,
Maneeprakorn Weerakanya,
Tangkuaram Tanin
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060340
Subject(s) - ascorbic acid , choline oxidase , chemistry , nuclear chemistry , detection limit , amperometry , biosensor , inorganic chemistry , electrode , electrochemistry , chromatography , organic chemistry , biochemistry , food science , acetylcholinesterase , enzyme
A novel metal composite material based on zirconium dioxide decorated gold nanoparticles (ZrO 2 @AuNPs), copper (I) oxide at manganese (IV) oxide (Cu 2 O@MnO 2 ) and immobilized choline oxidase (ChOx) onto a glassy carbon electrode (GCE) (ChOx/Cu 2 O@MnO 2 ‐ZrO 2 @AuNPs/GCE) has been developed for enhancing the electro‐catalytic property, sensitivity and stability of the amperometric choline biosensor. The ChOx/Cu 2 O@MnO 2 ‐ZrO 2 @AuNPs/GCE displayed an excellent electrocatalytic response to the oxidation of the byproduct H 2 O 2 from the choline catalyzed reaction, which exhibited a charge transfer rate constant ( K s ) of 0.97 s −1 , a diffusion coefficient value ( D ) of 4.50×10 −6 cm 2 s −1 , an electroactive surface area ( A e ) of 0.97 cm 2 and a surface concentration (γ) of 0.54×10 −8 mol cm −2 . The modified electrode also provided a wide linear range of choline concentration from 0.5 to 1,000.0 μM with good sensitivity (97.4 μA cm −2 mM −1 ) and low detection limit (0.3 μM). The apparent Michaelis‐Menten constant was found to be 0.08 mM with I max of 0.67 μA. This choline biosensor presented high repeatability (%RSD=2.9, n=5), excellent reproducibility (%RSD=2.9, n=5), long time of use (n=28 with %I>50.0 %) and good selectivity without interfering effects from possible electroactive species such as ascorbic acid, aspirin, amoxicillin, caffeine, dopamine, glucose, sucrose and uric acid. This optimal method was successfully applied for choline measurement in prepared human blood samples which demonstrated accurate and excellent reliability in the recovery range from 96.7 to 102.0 %.