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A Photoelectrochemical Immunosensor for Prostate Specific Antigen Detection Based on Graphdiyne Oxide Conjugated with Horseradish Peroxidase
Author(s) -
Wang Hao,
Xiao Jing,
Li Chunxiang,
Li Xiaofang,
Deng Keqin
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060296
Subject(s) - horseradish peroxidase , detection limit , graphene , conjugated system , biocompatibility , materials science , selectivity , photocurrent , linear range , oxide , nuclear chemistry , chemistry , nanotechnology , chromatography , catalysis , organic chemistry , optoelectronics , polymer , enzyme , metallurgy , composite material
Graphdiyne (GDY) was a novel flat material with sp and sp 2 hybridized carbon atoms. It exhibited good biocompatibility. The application of GDY in PEC immunosensor was very limited. Thus, a novel photoelectrochemical sensor for the sensitive detection of prostate specific antigen (PSA) was proposed by using GDY oxide (GDYO) conjugated with horseradish peroxidase (HRP) and secondary antibody for photocurrent signal inhibition. GDYO was prepared by oxidation of honeycomb‐like nanotubes composed of numerous GDY nanosheets. It showed high loading capacity for HRP and the catalytic activity of HRP could be remained. With reduced graphene oxide‐CdS (rGO‐CdS) as photoelectrochemical sensing platform, a sandwich‐type photoelectrochemical (PEC) immunosensor was thus fabricated. The immunosensor presented a wide linear concentration range of 10 fg mL −1 –20.0 ng mL −1 with a detection limit (LOD) of 3.5 fg mL −1 . Moreover, the PEC immunosensor displayed ideal reproducibility, stability, and selectivity, which was a promising platform for the detection of other important tumor targets.