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Kinetic Spectroelectrochemical Assay for Uric Acid in Human Urine
Author(s) -
Flowers Paul A.,
Dong Xin,
Bounds Jackson G.
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060251
Subject(s) - uric acid , urine , electrolysis , reagent , chromatography , chemistry , standard addition , detection limit , biochemistry , electrode , organic chemistry , electrolyte
A spectroelectrochemical (SEC) assay for uric acid in human urine has been developed that is essentially reagent‐free and involves relatively short analysis times of ∼12 min per specimen using a single‐point standard addition approach. The SEC method was demonstrated to be selective for uric acid in human urine, avoiding interference through both the usual control parameters (electrolysis potential and measurement wavelength) and the relatively slow kinetics of a post‐electrolysis reaction involving the oxidation product. Results from the SEC analysis of a split set of human urine specimens were well correlated to those obtained using a standard high‐performance liquid chromatography method, suggesting the feasibility of clinical applications.