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Highly Sensitive Impedimetric Biosensor Based on Thermolysin Immobilized on a GCE Modified with AuNP‐decorated Graphene for the Detection of Ochratoxin A
Author(s) -
Zinoubi Khaoula,
Chrouda Amani,
Soltane Raya,
AlGhamdi Youssef O.,
Garallah Almalki Sami,
Osman Gamal,
Barhoumi Houcine,
Jaffrezic Renault Nicole
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060247
Subject(s) - biosensor , detection limit , polyvinyl alcohol , ochratoxin a , thermolysin , graphene , polyethylenimine , chemistry , chromatography , colloidal gold , cellulose , materials science , nanoparticle , nuclear chemistry , nanotechnology , organic chemistry , mycotoxin , biochemistry , enzyme , transfection , trypsin , food science , gene
The fabrication of a thermolysin‐based biosensor capable of detecting ochratoxin A (OTA) from food samples is described. The electrochemical deposition of calcium cross‐linked cellulose film (CCLC) and gold nanoparticles (AuNPs) on graphene (GR) for modification of a glassy carbon electrode (GCE) is the first step. Then the thermolysin (TLN) enzyme in a polyvinyl alcohol (PVA)/polyethylenimine (PEI) matrix is immobilized. The impedimetric biosensor response is linear from 0.2 nM to 100 nM with a detection limit of 0.2 nM. The obtained stable and reproducible biosensor is then applied for the detection of OTA in spiked extracts from coffee beans.