Premium
Dual Responsive Disposable Electrode for the Enumeration of Escherichia coli in Whole Blood
Author(s) -
Panhwar Sallahuddin,
Ilhan Hasan,
Hassan Syeda Sara,
Zengin Adem,
Boyacı Ismail Hakkı,
Tamer Ugur
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060185
Subject(s) - enumeration , escherichia coli , electrode , cyclic voltammetry , immunomagnetic separation , colloidal gold , raman spectroscopy , chemistry , electrochemistry , surface enhanced raman spectroscopy , chromatography , nanoparticle , materials science , nuclear chemistry , analytical chemistry (journal) , nanotechnology , biochemistry , raman scattering , mathematics , physics , combinatorics , optics , gene
In this report, we have designed a dual responsive disposable electrode for the enumeration of Escherichia coli K12 (E. coli K12) . The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal‐organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro‐electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantify E. coli with a ranging from 10 1 to 10 7 cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.