Premium
Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker
Author(s) -
Xia YaMu,
Li MengYing,
Chen ChengLong,
Xia Meng,
Zhang Wen,
Gao WeiWei
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060039
Subject(s) - graphene , polyaniline , biosensor , materials science , electrode , nanofiber , oxide , electrochemistry , detection limit , nanotechnology , chemistry , polymer , chromatography , metallurgy , composite material , polymerization
A label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance ( R ct ), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10 −15 –1.0×10 −7 M) with a theoretical LOD of 3.01×10 −16 M (3 S/m ). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.