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An Impedimetric Aptasensor Based on a Novel Line‐Pad‐Line Electrode for the Determination of VEGF 165
Author(s) -
Feng Ying,
Xiao Shan,
Xiong Xingliang,
Wang Honglei,
Kong Fankai,
Li Yang,
Zhang Yan,
Chen Longcong
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060030
Subject(s) - aptamer , biosensor , electrode , detection limit , materials science , calibration curve , substrate (aquarium) , sensitivity (control systems) , electrical impedance , optoelectronics , nanotechnology , analytical chemistry (journal) , chemistry , electronic engineering , chromatography , electrical engineering , microbiology and biotechnology , oceanography , engineering , geology , biology
In this work, a novel, simple and label‐free line‐pad‐line electrode (LPLE) biosensor was developed for detection of vascular endothelial growth factor (VEGF 165 ). DNA aptamer was used as a recognition element for high specificity to VEGF 165 , and original LPLE as the substrate electrode for high sensitivity of the biosensor. This sensor was prepared by immobilizing anti‐VEGF 165 aptamers on the LPLE surface through gold‐sulfur (Au−S) bonding. Upon the addition of VEGF 165 , a large target‐induced conformational change in the surface‐immobilized aptamer was generated and caused variations in the interfacial properties,which led to a corresponding increase in the impedance magnitude of the LPLE. Finally, our results demonstrate that the calibration curve for VEGF 165 determination was linear over the range of 0.026‐31.4 fM with a detection limit as low as 0.017 fM.Additionally, our sensor was fabricated on printed circuit board (PCB) with a new electrode construction, and can potentially be implemented with the advantages of simplicity, low‐cost and easy mass production. Besides, considering its desirable sensitivity and specificity, the proposed use of LPLE provided a promising strategy for a wide variety of sensing applications.