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A Novel and Reusable Electrochemical Genosensor for Detection of Beef Adulteration
Author(s) -
Flauzino José M. R.,
Pimentel Eduardo L.,
Alves Lívia M.,
Madurro João M.,
BritoMadurro Ana G.
Publication year - 2021
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202060029
Subject(s) - chemistry , oligonucleotide , chromatography , dna , biochemistry
This work describes the construction of a genosensor based on a graphite electrode modified with an reduced graphene oxide/poly(3‐hidroxybenzoic acid) nanocomposite with an specific DNA oligonucleotide for detection of cattle mitochondrial DNA, in order to certify beef purity. Electrochemical and morphological analyses indicate that the genosensor allows duplex formation with the DNA of pure sample of beef lysate. The genosensor was selective, identifying up to 1 % (w/w) of pork in beef samples, showing good reproducibility and stability within six weeks of storage, and can be reused four times, being a great tool for the evaluation of beef purity, with application in the meat production and marketing chain.