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Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum
Author(s) -
Ribeiro Sandro H. D.,
Alves Lívia M.,
Flauzino José M. R.,
Moço Anna C. R.,
Segatto Mônica S.,
Silva Jonathan P.,
Borges Lizandra F. A.,
Madurro João M.,
Madurro Ana G. B.
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.202000043
Subject(s) - differential pulse voltammetry , dielectric spectroscopy , graphene , electrochemistry , electrode , chemistry , scanning electron microscope , cyclic voltammetry , analyte , materials science , chromatography , nanotechnology , analytical chemistry (journal) , composite material
This work describes the preparation of a reusable electrochemical immunosensor for detection of C‐reactive protein (CRP), based in modified carbon electrode with reduced graphene oxide and polytyramine as strategy for anchoring the anti‐CRP probe. This immunosensor was able to distinguish different concentrations of CRP target in human serum, by differential pulse voltammetry, showing a linear response from 1.09 to 100 μg L −1 . The performance of the immunosensor allowed its application in four consecutive assays, without activity loss. Analyses by atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy corroborate with voltammetric analysis. The immunosensor showed selectivity, stability and reusability, being a promising platform for point‐of‐care quantification of CRP.