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Involvement of Proton Transfer for Carbon Dioxide Reduction Coupled with Extracellular Electron Uptake in Shewanella oneidensis MR‐1
Author(s) -
La Cava Eugenio,
Guionet Alexis,
Saito Junki,
Okamoto Akihiro
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900686
Subject(s) - shewanella oneidensis , electrosynthesis , chemistry , microbial fuel cell , electron transfer , electrochemistry , cathodic protection , shewanella , inorganic chemistry , formic acid , cathode , electron transport chain , electrode , biochemistry , bacteria , photochemistry , anode , biology , genetics
Microbial biosynthesis of hydrocarbon from CO 2 reduction driven by electron uptake process from the cathodic electrode has gained intensive attention in terms of potential industrial application. However, a lack of a model system for detailed studies on the mechanism of the CO 2 reduction hinders the improvement in efficiency for microbial electrosynthesis. Here, we examined the mechanism of microbial CO 2 reduction at the cathode by a well‐described microbe for extracellular electron uptake, Shewanella oneidensis MR‐1, capable of reducing gaseous CO 2 to produce formic acid. Using whole‐cell electrochemical assay, we observed stable cathodic current production at −0.65 V vs Ag/AgCl KCl sat. associated with the introduction of CO 2 . The observed cathodic current was enhanced by the addition of 4 μM riboflavin, which specifically accelerates the electron uptake process of MR‐1 by the interaction to its outer‐membrane c ‐type cytochromes. The significant impact of an uncoupler agent and a mutant strain of MR‐1 lacking sole F‐type ATPase suggested the importance of proton import to the cytoplasm for the cathodic CO 2 reduction. The present data suggest that MR‐1 potentially serves as a model system for microbial electrosynthesis from CO 2 .