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Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples
Author(s) -
MohammadRezaei Rahim,
Golmohammadpour Mahdi
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900592
Subject(s) - amperometry , biosensor , nanocomposite , cyclic voltammetry , electrode , copper , materials science , nicotinamide adenine dinucleotide , horizontal scan rate , detection limit , oxide , copper oxide , scanning electron microscope , nuclear chemistry , analytical chemistry (journal) , electrochemistry , carbon paste electrode , inorganic chemistry , chemistry , nanotechnology , chromatography , nad+ kinase , metallurgy , composite material , organic chemistry , enzyme
In this study, a sensitive nicotinamide adenine dinucleotide (NADH) biosensor based on Au‐Copper oxide nanocomposite modified carbon ceramic electrode (Au−CuO/CCE) was introduced. The developed NADH biosensor was prepared by controlled electrodeposition of copper and Au nanoparticles on the surface of a renewable CCE and was turned to Au−CuO/CCE by cycling the potential in alkaline media. The prepared electrode was carefully characterized with scanning electron microscopy, X‐ray diffraction, atomic force microscopy and cyclic voltammetry techniques. According to scan rate study, surface coverage (Γ) of the fabricated Au−CuO/CCE was calculated to be 1.54×10 −8 mol cm −2 which was 3 time more than CuO/CCE. The fabricated electrode is well stable which could be reliably utilized for the determination of NADH with amperometry technique over the concentration range of 1–29 μM with sensitivity and detection limit (S/N=3) of 0.1025 μA μM −1 and 0.09 μM respectively. The prepared biosensor was used for NADH determination in serum samples with fast response time and satisfactory analytical results.