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A Laccase/Chitosan‐Lambda‐Carrageenan Based Voltammetric Biosensor for Phenolic Compound Detection
Author(s) -
Chakroun Galai Hajer,
Rassas Ilhem,
Namour Philippe,
Bonhomme Anne,
Raimondi Gaetan,
Besbes Hentati Salma,
JaffrezicRenault Nicole
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900531
Subject(s) - biosensor , chitosan , catechol , detection limit , chemistry , laccase , chromatography , nuclear chemistry , repeatability , cyclic voltammetry , hydroquinone , electrochemistry , organic chemistry , electrode , biochemistry , enzyme
In this contribution, a new concept of voltammetric catechol biosensor, based on the encapsulation of laccase (LAC) in a chitosan+lambda‐carrageenan (CHIT+CAR) polyelectrolyte complex (PEC) employing a simple coacervation process is presented. Chitosan (CHIT) was prepared from α‐chitin extracted from shrimp shells and lambda‐carrageenan (CAR) was extracted from red algae, both polysaccharides and PEC being characterized using FTIR spectrometry and electrochemistry. Cyclic voltammetry was utilized to determine the analytical features of the laccase (LAC) biosensor for catechol detection. The linear range was from 10 −20 M to 10 −14 M with a sensitivity of 1.55 mA/p[catechol] and a limit of detection of 3×10 −21 M.The laccase biosensor exhibits good repeatability (RSD 2.38 %) and stability (four weeks). The developed biosensor was tested by applying it to the evaluation of the total polyphenolic content in natural oil samples.