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Biomolecular Release Stimulated by Electrochemical Signals at a Very Small Potential Applied
Author(s) -
Masi Madeline,
Bollella Paolo,
Katz Evgeny
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900377
Subject(s) - electrode , electrochemistry , molecule , adsorption , trigonelline , dna , fluorescence , nanoparticle , chemistry , chemical engineering , nanotechnology , combinatorial chemistry , materials science , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , physics , quantum mechanics , engineering
DNA release electrochemically stimulated by applying −10 mV on the modified electrode was studied. The release process was based on the local (interfacial) pH change produced upon H 2 O 2 reduction electrocatalyzed by the immobilized microperoxidase‐11. SiO 2 nanoparticles attached to the electrode surface and functionalized with trigonelline and boronic acid species changed their electrical charge from positive to negative upon the interfacial pH change, thus allowing electrostatic adsorption of negatively charged DNA on the positive interface and then its repulsion/release from the negative interface. The loaded/released DNA molecules were labeled with a fluorescent dye to allow easy detection of the released DNA molecules. The important feature of the developed system is the controlled DNA release upon applying very small electrical potential on the modified electrode.