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Amperometric Biosensors for Tyramine Determination Based on Graphene Oxide and Polyvinylferrocene Modified Screen‐printed Electrodes
Author(s) -
Erden Pınar Esra,
Erdoğan Zehra Özden,
Öztürk Funda,
Koçoğlu İrem Okman,
Kılıç Esma
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900369
Subject(s) - biosensor , tyramine , cadaverine , graphene , diamine oxidase , chemistry , cyclic voltammetry , amperometry , nuclear chemistry , analytical chemistry (journal) , electrode , putrescine , materials science , chromatography , electrochemistry , nanotechnology , organic chemistry , biochemistry , enzyme
A comparison of the analytical characteristics of two tyramine biosensors, based on graphene oxide (GRO) and polyvinylferrocene (PVF) modified screen‐printed carbon electrodes (SPCE), is reported. Diamine oxidase (DAOx) or monoamine oxidase (MAOx) was immobilized onto the PVF/GRO modified SPCE to fabricate the biosensors. Surface characteristics and electrochemical behaviour of the modified SPCEs were investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM), energy dispersive X‐ray spectroscopy (EDX) and cyclic voltammetry (CV). Electrode surface composition and experimental variables such as pH and working potential were optimized in order to ensure a high performance. Under optimum experimental conditions, both DAOx/PVF/GRO/SPCE and MAOx/PVF/GRO/SPCE biosensors exhibited wide linear dynamic ranges for tyramine from 9.9×10 −7 to 1.2×10 −4 M and from 9.9×10 −7 to 1.1×10 −4 M, respectively. MAOx/PVF/GRO/SPCE biosensor showed higher sensitivity (11.98 μA mM −1 ) for tyramine determination than the DAOx/PVF/GRO/SPCE biosensor (7.99 μA mM −1 ). The substrate specifity of the biosensors to other biogenic amines namely histamine, putrescine, spermine, spermidine, tryptamine, β‐phenylethylamine and cadaverine was also investigated. The developed biosensors were successfully used for tyramine determination in cheese sample.