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Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME)
Author(s) -
Basu Nivedita,
Ho Thu Huong,
Guillon FrançoisXavier,
Zhang Yuanyuan,
Bigey Pascal,
Navakanta Bhat,
Bedioui Fethi,
Lazerges Mathieu
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900357
Subject(s) - ultramicroelectrode , electron transfer , electrochemistry , dna , chemistry , adsorption , coupling (piping) , chemical physics , analytical chemistry (journal) , biophysics , materials science , cyclic voltammetry , electrode , photochemistry , biochemistry , chromatography , biology , metallurgy
A new electrochemical hybridization transduction pathway, obtained by coupling electrochemical adsorption and long‐range electron transfer through double‐stranded DNA, was investigated using ultramicroelectrode (UME). The results show that long‐range electron transfer does not occurs exclusively throws well‐packed and organized self‐assembled DNA monolayers. This approach is used to investigate long‐range electron transfer properties of both single‐ and double‐ stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non‐labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed.