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A Simple, Cost‐effective, and Environmentally Friendly Method for Determination of Ciprofloxacin in Drugs and Urine Samples Based on Electrogenerated Chemiluminescence
Author(s) -
Pavão e Pavão Daniele,
Nascimento Botelho Chirlene,
Nunes Fernandes Ridvan,
Costa dos Santos Clenilton,
Santos Damos Flavio,
Cássia Silva Luz Rita
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900355
Subject(s) - ciprofloxacin , detection limit , chemiluminescence , environmentally friendly , linear range , urine , chromatography , chemistry , materials science , antibiotics , ecology , biochemistry , biology
Ciprofloxacin is an antibiotic that belongs to the class of drugs known as quinolones and it is frequently used to treat a variety of bacterial infections. The present work aims the development of a simple, cost‐effective, and environmentally friendly method for the determination of ciprofloxacin in drugs and artificial urine samples due to the high importance of this antibiotic for the human health. The proposed method is based on the electrogenerated chemiluminescence (ECL) resulting from the reaction between the ciprofloxacin and the tris(2,2′‐bipyridyl)ruthenium(II) complex. This method exploits a screen‐printed carbon electrode positioned in an ECL cell with capacity to 50 μL of electrolytic solution. The ECL intensity was monitored with the aid of a photodiode. The ECL signal was simultaneously registered to the voltammetric measurements. Under optimized experimental conditions, the ECL method presented a linear response range for ciprofloxacin between 0.5 and 500 μmol L −1 (or 0.0005 and 0.5 mmol L −1 ). The proposed method presented a detection limit of 0.5 μmol L −1 and it was successfully applied for the ciprofloxacin determination in drugs and artificial urine samples, with good accuracy and precision.