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Deuterium Isotope Effects Upon the Redox‐switching of the Viscosity of DNA Layers Observed by Electrochemical Quartz Crystal Micro‐balance
Author(s) -
Galagedera Sarasi K. K.,
Flechsig GerdUwe
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900350
Subject(s) - redox , electrochemistry , chemistry , quartz crystal microbalance , kinetic isotope effect , molecule , inorganic chemistry , crystal (programming language) , monolayer , electrode , analytical chemistry (journal) , deuterium , organic chemistry , adsorption , biochemistry , quantum mechanics , computer science , programming language , physics
Abstract We report on H/D isotope effects observed upon quick redox‐switching of the viscoelasticity of self‐assembled monolayers of single‐stranded DNA (ssDNA‐SAM) observed by electrochemical quartz‐crystal micro‐balance (EQCM) of three redox‐active small molecules that travel through the DNA layer on gold electrodes. We have recently reported hexammine cobalt(III) (CoHex) to have the largest voltammetric isotope effect while hexammine ruthenium(III) (RuHex) does not show this effect. Daunomycin, on the other hand showed a significant redox potential shift up to −80 mV. A thin‐layer model may explain this voltammetric behavior. RuHex covers the negatively charged DNA strand and provides considerable conductivity, while CoHex and daunomycin do not. Latest results regarding the reproducible frequency responses indicate considerable isotope effects also in EQCM measurements depending on the redox molecule interacting with the ssDNA‐SAM. These effects will provide new opportunities in drug screening and studies of DNA damage by toxic chemicals.