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Electrochemical Determination and in silico Studies of Fludarabine on NH 2 Functionalized Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode
Author(s) -
DoganTopal Burcu,
Bakirhan Nurgul K.,
Tok Tugba Taskin,
Ozkan Sibel A.
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900347
Subject(s) - cyclic voltammetry , differential pulse voltammetry , fludarabine , carbon nanotube , voltammetry , electrochemistry , materials science , analytical chemistry (journal) , glassy carbon , chemistry , electrode , organic chemistry , nanotechnology , medicine , surgery , chemotherapy , cyclophosphamide
Abstract A sensitive voltammetric technique has been developed for the determination of Fludarabine using amine‐functionalized multi walled carbon nanotubes modified glassy carbon electrode (NH 2 ‐MWCNTs/GCE). Molecular dynamics simulations, an in silico technique, were employed to examine the properties including chemical differences of Fludarabine‐ functionalized MWCNT complexes. The redox behavior of Fludarabine was examined by cyclic, differential pulse and square wave voltammetry in a wide pH range. Cyclic voltammetric investigations emphasized that Fludarabine is irreversibly oxidized at the NH 2 ‐MWCNTs/GCE. The electrochemical behavior of Fludarabine was also studied by cyclic voltammetry to evaluate both the kinetic (k s and E a ) and thermodynamic (ΔH, ΔG and ΔS) parameters on NH 2 ‐MWCNTs/GCE at several temperatures. The mixed diffusion‐adsorption controlled electrochemical oxidation of Fludarabine revealed by studies at different scan rates. The experimental parameters, such as pulse amplitude, frequency, deposition potential optimized for square‐wave voltammetry. Under optimum conditions in phosphate buffer (pH 2.0), a linear calibration curve was obtained in the range of 2×10 −7 M–4×10 −6 M solution using adsorptive stripping square wave voltammetry. The limit of detection and limit of quantification were calculated 2.9×10 −8 M and 9.68×10 −8 M, respectively. The developed method was applied to the simple and rapid determination of Fludarabine from pharmaceutical formulations.