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Electrochemical Detection of Vascular Endothelial Growth Factor by Molecularly Imprinted Polymer
Author(s) -
BozalPalabiyik Burcin,
Lettieri Mariagrazia,
Uslu Bengi,
Marrazza Giovanna
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900185
Subject(s) - molecularly imprinted polymer , detection limit , cyclic voltammetry , electrode , materials science , vascular endothelial growth factor , scanning electron microscope , electrochemistry , chromatography , chemistry , vegf receptors , selectivity , biochemistry , cancer research , medicine , composite material , catalysis
In this study, we report the development of a sensitive label‐free impedimetric sensor based on molecularly imprinted polymer (MIP) as biomimetic receptor coupled with screen‐printed electrodes (SPEs) for the detection of vascular endothelial growth factor (VEGF). Firstly, electropolymerization of o‐phenylenediamine (o‐PD) in the presence of VEGF molecule by cyclic voltammetry was performed onto graphite screen‐printed electrodes. The solvent extraction of the target was then carried out. The MIP based sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Using optimized experimental conditions, the single‐use MIP‐based sensor showed a good analytical performance for VEGF detection from 20 to 200 pg mL −1 with limit of detection of 0.08 pg mL −1 . Finally, the developed MIP‐based sensor in human serum samples was also tested.