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Diazirine‐functionalized Nanostructured Platform for Enzymes Photografting and Electrochemical Biosensing
Author(s) -
Stoytcheva Margarita,
Zlatev Roumen,
Velkova Zdravka,
Gochev Velizar,
Ayala Alan,
Montero Gisela,
Valdez Benjamín
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900086
Subject(s) - diazirine , biosensor , chemistry , analyte , differential pulse voltammetry , glassy carbon , tyrosinase , electrochemistry , combinatorial chemistry , cyclic voltammetry , nanotechnology , electrode , chromatography , organic chemistry , materials science , enzyme , photochemistry , biochemistry
A simple technique for the construction of a versatile diazirine‐functionalized nanostructured platform for enzymes photografting and electrochemical biosensing was proposed in this work. The feasibility of the approach was proved by photo crosslinking of an enzyme, tyrosinase, to diazirine‐activated aminated carbon nanotubes coated glassy carbon electrode. The analytical performances of the realized biosensor were evaluated employing catechol as analyte. Then the sensor based on the diazirine‐functionalized nanostructured platform with photografted tyrosinase was applied together with the high resolution technique Differential Alternative Pulses Voltammetry for dopamine determination in the linear concentration range of 5–25 μmol L −1 in the presence of interfering agents as uric acid up to its 100‐fold excess.