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Studies on the Affinity‐based Biosensors for Electrochemical Detection of HER2 Cancer Biomarker
Author(s) -
Jarczewska Marta,
Trojan Anita,
Gągała Małgorzata,
Malinowska Elżbieta
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201900041
Subject(s) - aptamer , chemistry , biosensor , chronoamperometry , polyclonal antibodies , biomarker , cancer biomarkers , antibody , target protein , ferrocyanide , electrode , cancer , chromatography , electrochemistry , nanotechnology , combinatorial chemistry , microbiology and biotechnology , cyclic voltammetry , biochemistry , materials science , biology , medicine , immunology , gene
As cancer diseases are the second main cause of death it is necessary to elaborate fast and efficient early diagnosis methods for their detection. One of the possibilities is the analysis of protein biomarkers, which abnormal concentration in physiological fluids might be an indication of cancer disease progression. Herein, we present the studies on the development of affinity‐based biosensors for electrochemical detection of HER2 protein, which is a common biomarker of breast cancer. The main objective was to verify the possibility of fabrication of HER2‐specific hybrid aptamer‐polyclonal antibody and antibody‐based sandwich sensing layer on gold electrode surface. The effectiveness of each electrode modification step was confirmed using voltammetric and impedimetric techniques in the presence of ferri/ferrocyanide redox couple. It was observed that hybrid construct was unlikely to be formed on the gold electrode due to a higher affinity of secondary polyclonal antibody towards target protein, which resulted in the separation of HER2‐antibody complex from the electrode surface. On the contrary, an antibody‐based sandwich receptor layer allowed for protein discrimination in the range from 1 to 100 ng mL −1 by the application of TMB/H 2 O 2 system and chronoamperometry detection technique. Though, the occurrence of interactions between interfering proteins and antibody‐based layer was noted, it led to at least two times smaller current responses than for HER2 protein.