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Cover Feature: Impedance Detection of 3‐Phenoxybenzoic Acid with a Noncompetitive Two‐site Phage Anti‐immunocomplex Assay (Electroanalysis 11/2018)
Author(s) -
Pali Madhavi,
Bever Candace R. S.,
Vasylieva Natalia,
Hammock Bruce D.,
Suni Ian I.
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201881103
Subject(s) - bacteriophage , polyclonal antibodies , immunoassay , ternary operation , cover (algebra) , ternary complex , chemistry , combinatorial chemistry , antibody , biology , biochemistry , escherichia coli , computer science , enzyme , mechanical engineering , engineering , immunology , gene , programming language
Impedance Detection of 3‐Phenoxybenzoic Acid with a Noncompetitive Two‐site Phage Anti‐immunocomplex Assay The cover picture shows formation of a ternary complex between M13 bacteriophage, 3‐phenoxybenzoic acid (3‐PBA), and the polyclonal antibody to 3‐PBA. This use of bacteriophage circumvents that usual limitations for small molecule sandwich immunoassays, where small molecules are not large enough to contain two antibody binding epitopes. In this article, we report the first bacteriophage‐assisted sandwich immunoassay, which forms this ternary complex, utilizing signal transduction by electrochemical impedance spectroscopy. More details are discussed in the article by Madhavi Pali, Candace R. S. Bever, Natalia Vasylieva, Bruce D. Hammock, and Ian I. Suni. DOI: 10.1002/elan.201800457