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Plasma‐functionalized Highly Aligned CNT‐based Biosensor for Point of Care Determination of Glucose in Human Blood Plasma
Author(s) -
Azimi Shamim,
Farahani Ali,
Sereshti Hassan
Publication year - 2020
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800895
Subject(s) - materials science , cyclic voltammetry , glucose oxidase , analytical chemistry (journal) , fourier transform infrared spectroscopy , electrode , polyaniline , biosensor , raman spectroscopy , carbon nanotube , detection limit , attenuated total reflection , substrate (aquarium) , scanning electron microscope , plasma enhanced chemical vapor deposition , chemical vapor deposition , chemical engineering , nanotechnology , electrochemistry , chemistry , chromatography , optics , polymer , oceanography , engineering , composite material , polymerization , geology , physics
In this study, a new method for modification of vertically aligned carbon nanotube arrays (VACNTs) for selective detection of glucose was developed. VACNTs were grown by chemical vapor deposition method on a silicon substrate deposited with alumina as a buffer layer and iron as a catalyst using radio frequency (RF) sputtering and electron beam evaporation, respectively. The surface of the electrode was modified with electrodeposition of polyaniline (PANI) followed by covalent attachment of glucose oxidase (GOx). The electrode was characterized using field emission scanning electron microscopy (FESEM), micro‐Raman spectroscopy, and attenuated total reflectance Fourier transform infrared spectrometer (ATR‐FTIR) techniques. Cyclic voltammetry and differential pulse voltammetry were used to investigate the electrochemical behavior of the electrode. The fabricated electrode was successfully employed as a point‐of‐care (POC) biosensor for the detection of glucose in human blood plasma. The detection limit was 1.1 μM, and the sensitivity was 620 μA mM −1 cm −2 at the linear range of 2–426 μM.