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Multiplexed Electrochemical Cancer Diagnostics With Automated Microfluidics
Author(s) -
Mercer Conan,
Jones Abby,
Rusling James F.,
Leech Dónal
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800632
Subject(s) - microcontroller , multiplexing , horseradish peroxidase , microfluidics , embedded system , computer hardware , automation , immunoassay , computer science , throughput , protocol (science) , multiplex , arduino , nanotechnology , materials science , chemistry , bioinformatics , engineering , wireless , mechanical engineering , medicine , telecommunications , biochemistry , enzyme , alternative medicine , pathology , antibody , immunology , biology
Microfluidic platforms can lead to miniaturisation, increased throughput and reduced reagent consumption, particularly when the processes are automated. Here, a programmable microcontroller is used for automation of a microfluidic platform configured to electrochemically determine the levels of 8 proteins simultaneously in complex liquid samples. The platform system is composed of a programmable Arduino microcontroller that controls inexpensive valve actuators, pump, magnetic stirrer and electronic display. The programmable microcontroller results in repeatable timing for each step in a complex assay protocol, such as sandwich immunoassays. Application of the platform is demonstrated using a multiplexed electrochemical immunoassay based on capture at the electrode surface of magnetic particles labelled with horseradish peroxidase and detection antibody. The multiplexed assay protocol is completed in less than 30 mins and results in detection of eight proteins associated with prostate cancer. The approach presented can be used to automate and simplify high‐throughput screening campaigns, such as detection of multiple biomarkers in patient samples.