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Zero‐valent Iron Nanoparticles Modified Screen‐printed Electrode for FIA or HPLC Amperometric Detection of Metronidazole in Pharmaceutical Formulations
Author(s) -
Sanchez Cesar A.,
Rodríguez Jose A.,
PaezHernandez M. Elena,
Santos Eva M.,
Castrillejo Yolanda
Publication year - 2019
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800617
Subject(s) - amperometry , detection limit , repeatability , chromatography , high performance liquid chromatography , flow injection analysis , analyte , chemistry , electrode , electroanalytical method , analytical chemistry (journal) , electrochemistry , potentiometric titration
A zero‐valent iron nanoparticles modified electrode was employed as an amperometric detector in flow conditions to determine metronidazole and 2‐methyl‐5‐nitroimidazole in pharmaceutical formulations. Flow injection analysis at −0.6 V ( vs . Ag) achieved limits of detection of 2 and 6 μM for metronidazole and 2‐methyl‐5‐nitroimidazole, respectively, but the analysis was not discriminative between the two compounds. When reverse phase high performance liquid chromatography was applied previous to the electrochemical detection both analytes could be analysed simultaneously. However, the limits of detection slightly increased (10 μM) as a consequence of the use of an organic solvent and a lower sample volume. The relative standard deviation of analytical repeatability was <4.0 % in both techniques. The methods were validated by comparing the results obtained from the analysis of commercial samples with those provided by HPLC‐DAD and no significant differences were detected. Results probed that the modified electrode was a successful tool in the FIA and HPLC analysis of nitro compounds.