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An Electrochemical Immunosensor with Graphene‐Oxide‐Ferrocene‐based Nanocomposites for Hepatitis B Surface Antigen Detection
Author(s) -
Zhao Feijun,
Bai Yan,
Zeng Ruosheng,
Cao Liangli,
Zhu Jianming,
Han Guocheng,
Chen Zhencheng
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800476
Subject(s) - hbsag , graphene , materials science , detection limit , biocompatibility , oxide , ferrocene , nanochemistry , linear range , electrochemistry , nanotechnology , hepatitis b virus , chemistry , virus , chromatography , electrode , virology , medicine , metallurgy
The detection of hepatitis B surface antigen (HBsAg), a coat protein secreted by hepatitis B virus, is a common method for confirming infection in early diagnosis. A new composite of graphene oxide‐ferrocene‐chitosan (GO‐Fc‐CS) was synthesized and used to develop an electrochemical immunosensor with a GO‐Fc‐CS/Au‐nanoparticle layer film for HBsAg detection. The modified layer film exhibited not only enhanced electron conductivity, but also strong reversible redox signal for current changes, excellent biocompatibility, and good film‐forming ability for binding a large number of antibodies. The immunosensor exhibits a wide linear range from 0.05 to 150 ng/mL and detection limit of 0.01 ng/mL for HBsAg. The excellent performance in HBsAg detection suggests that the proposed immunosensor has potential application in clinical diagnosis.