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Electroactive, Mediating and Thermosensitive Microgel Useful for Covalent Entrapment of Enzymes and Formation of Sensing Layer in Biosensors
Author(s) -
Marcisz Kamil,
Kaniewska Klaudia,
Mackiewicz Marcin,
Nowinska Anna,
Romanski Jan,
Stojek Zbigniew,
Karbarz Marcin
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800459
Subject(s) - glucose oxidase , biosensor , amperometry , acrylate , microreactor , glassy carbon , chemical engineering , materials science , immobilized enzyme , coating , layer (electronics) , electrode , chemistry , polymer , nanotechnology , electrochemistry , organic chemistry , enzyme , cyclic voltammetry , catalysis , monomer , engineering
Thermosensitive microgel particles based on the polymeric network synthesized from N ‐isopropylacrylamide, sodium acrylate and N , N′ ‐bisacryloylcystine have been functionalized with aminoferrocene, a mediatior, and a model enzyme‐glucose oxidase (GOx). The product was very stable. A thin coating of that material has been successfully attached to the glassy carbon electrode surface to form a sensing layer. A temperature increase to a value higher than the volume phase transition temperature led to a substantial increase in the amperometric signal. That behavior was well switchable. The amperometric signal was linearly dependent on glucose concentration in the physiological concentration range. It appeared that the synthesized and functionalized microgel was a kind of microreactor and might be very useful for the construction of the platforms that can enhance the activity of enzymes in biosensors.