Premium
Stripe‐shaped Electrochemical Biosensor for Organophosphate Pesticide
Author(s) -
Celebanska Anna,
Jedraszko Justyna,
Lesniewski Adam,
Jubete Elena,
Opallo Marcin
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800406
Subject(s) - biosensor , organophosphate , detection limit , acetylcholinesterase , chemistry , pesticide , electrode , immobilized enzyme , electrochemistry , chromatography , nanotechnology , enzyme , materials science , organic chemistry , biochemistry , biology , agronomy
Detection of organophosphate pesticides is indispensable to improve the quality of the water and therefore required by the European Union law. Here, we propose a novel architecture of a pesticide biosensor where the enzyme and the electrode are placed into neighboring zones in the stripe form. This separation permits to use the optimal conditions for their immobilization resulting in undisrupted mechanical stability of the biosensor. The enzyme, acetylcholinesterase, generates thiocholine which is further oxidized at the carbon nanoparticles film electrode, exhibiting high electrocatalytic activity. The whole device was tested as a biosensor for malaoxon employing a wild or recombinant acetylcholinesterase. The latter allowed for incubation time decrease down to 10 minutes and lowered the detection limit to 0.25 nM. Moreover, the encapsulated enzyme activity was visualised by a scanning electrochemical microscopy.