Premium
Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator
Author(s) -
Vasconcelos Luydson R. S.,
Moura Patricia,
Dutra Rosa F.
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800377
Subject(s) - amplicon , genotype , single nucleotide polymorphism , microbiology and biotechnology , biology , sybr green i , snp , genetics , chemistry , gene , polymerase chain reaction
A genosensor for typing the IL28B single nucleotide polymorphism (rs12979860), an important marker predictive of the treatment response to hepatitis C (HCV) infection was developed. The electrochemical sensor was based on a nanohybrid poly‐pyrrole‐carbon multiwall nanotubes film functionalized with minor groove binding (MGB) oligonucleotides probes to allele C of the IL28B . Individuals with genotype CC are more likely to achieve the virus clearance after HCV treatment (60 %), while homozygous TT (variant) is associated to a poor therapy response (20 %). A conventional SYBR green PCR was performed to obtain amplicons of CC and TT genotypes to test the DNA binding response of an MBG‐genosensor device. The redox response was monitored as hybridization match arose between probes and amplicons. The results showed a significant increase in the current redox peaks after TT genotype amplicons incubations in comparison to CC genotypes, ascribed to the weak SYBR Green intercalation between strands, and exposing the its reactive groups, favouring an increased electrocatalytic response. In conclusion, it was presented a new architecture of a genosensor with On‐Off polarity exploring the high specificity and sensitivity of MGB‐probes with promise application for single nucleotide polymorphism, using Syber Green as electrochemical indicator.