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Carbon‐nanotube Modified Screen‐printed Electrode for the Simultaneous Determination of Nitrite and Uric Acid in Biological Fluids Using Batch‐injection Amperometric Detection
Author(s) -
Caetano Larissa P.,
Lima Ana P.,
Tormin Thiago F.,
Richter Eduardo M.,
Espindola Foued S.,
Botelho Françoise V.,
Munoz Rodrigo A. A.
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800189
Subject(s) - amperometry , detection limit , differential pulse voltammetry , chromatography , chemistry , calibration curve , linear range , dilution , matrix (chemical analysis) , analytical chemistry (journal) , electrode , electrochemistry , cyclic voltammetry , physics , thermodynamics
A portable electroanalytical system applied for rapid and simultaneous determination of uric acid (UA) and nitrite (NIT) in human biological fluids (urine, saliva and blood) is reported. The system is based on batch‐injection analysis with multiple‐pulse amperometric (BIA‐MPA) detection using screen‐printed electrodes (SPEs) modified with multi‐walled carbon nanotubes. Sample dilution in optimized electrolyte (0.1 mol L −1 Britton‐Robinson buffer pH 2) followed by injection of 100 μL on the electrode surface using an electronic micropipette is performed. UA is detected at +0.45 V and both UA+NIT at +0.70 V. Linear calibration plots for UA and NIT were obtained over the range of 1–500 μmol L −1 with detection limits of 0.05 and 0.06 μmol L −1 , respectively. For comparison, a differential‐pulse voltammetric (DPV) method was optimized, and linear calibration plots for UA and NIT were obtained over range of 1–30 μmol L −1 and 1–40 μmol L −1 with detection limits of 0.1 and 0.3 μmol L −1 , respectively. BIA‐MPA is highly precise (RSD<1.3 %), fast (160 h −1 ) and free from sample‐matrix interferences as recovery values ranged from 77 to 121 % for spiked samples (short contact time of sample aliquot with SPE). Contrarily, recovery tests conducted using DPV did not provide adequate recovery values (>150 %), probably due to the longer contact time of the SPE with the biological samples during analysis leading to a severe interference of sample matrices.