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Comparison of NAD with NADP‐dependent Glutamate Dehydrogenase, and CNT with rGO‐modified Electrodes, for the Construction of Glutamate Sensors
Author(s) -
Sato Katsuhiko,
Kamijo Toshio,
Takahashi Shigehiro,
Sato Takaya
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800160
Subject(s) - nicotinamide adenine dinucleotide , glutamate dehydrogenase , nad+ kinase , nicotinamide adenine dinucleotide phosphate , glutamate receptor , electrode , graphene , chemistry , glutamate synthase , nicotinamide , glassy carbon , diaphorase , inorganic chemistry , dehydrogenase , nuclear chemistry , enzyme , biochemistry , materials science , electrochemistry , nanotechnology , cyclic voltammetry , oxidase test , receptor
Glutamate sensors were prepared using nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP)‐dependent glutamate dehydrogenase (GDH). Glutamate was determined from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH. These two types of GDH enzymes were deposited separately on carbon nanotube (CNT) and reduced graphene oxide (rGO)‐modified glassy carbon electrodes, in which CNT and rGO were used to improve the oxidation capacity of the electrodes. An anodic peak current in response to 1 mM glutamate of glutamate sensors comprising each of these four combinations were compared under the same conditions. The glutamate response was the highest when NAD‐dependent GDH was deposited on the rGO‐modified electrode.