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Fe 2 V 4 O 13 Nanoparticles Based Electrochemical Sensor for the Simultaneous Determination of Guanine and Adenine at Nanomolar Concentration
Author(s) -
Adarakatti Prashanth Shivappa,
Mahanthappa Mallappa,
H Eranjaneya,
Siddaramanna Ashoka
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800124
Subject(s) - detection limit , differential pulse voltammetry , electrochemistry , electrochemical gas sensor , cyclic voltammetry , guanine , analytical chemistry (journal) , stripping (fiber) , electrode , voltammetry , chemistry , nanoparticle , nuclear chemistry , materials science , chromatography , nanotechnology , nucleotide , biochemistry , composite material , gene
A simple strategy has been proposed for the simultaneous quantification of guanine (GU) and adenine (AD) using Fe 2 V 4 O 13 nanoparticles (Fe 2 V 4 O 13 NPs) modified carbon paste electrode (Fe 2 V 4 O 13 NPs/CPE) in phosphate buffer solution (PBS). The Fe 2 V 4 O 13 NPs were prepared by a simple solution combustion method where sucrose was used as a fuel. The electrochemical behavior of GU and AD at the electrochemical interface has been studied by using cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV). The results illustrate that the Fe 2 V 4 O 13 NPs shows enhanced electrocatalytic activity and voltammetric response towards GU and AD. The proposed sensor showed linearity between the concentration 0.5 and 60 μM with limit of detection (LOD) 32 and 37 nM for GU and AD respectively. The sensitivity towards GU and AD were respectively found to be 1.393 and 1.851 μA/μM. Further, the proposed electrochemical sensor has been successfully employed to determine GU and AD contents in milk powder and calf thymus DNA samples.