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Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis
Author(s) -
Kanat Erkin,
Eksin Ece,
Karacicek Bilge,
Eraç Yasemin,
Erdem Arzum
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800064
Subject(s) - guanine , nucleic acid , agarose gel electrophoresis , dielectric spectroscopy , carbon nanotube , chemistry , agarose , electrochemistry , electrophoresis , electrode , nuclear chemistry , dna , materials science , analytical chemistry (journal) , chromatography , nanotechnology , biochemistry , nucleotide , gene
Anticancer drug Dacarbazine (DCB) has been used for the treatment for metastatic malignant melanoma. In our study, the biomolecular interaction between DCB and DNA was investigated electrochemically for the first time using single‐walled carbon nanotube (SWCNT) modified disposable pencil graphite electrode (PGEs). The SWCNT modified PGEs were optimized for advanced DNA sensing based on the response related to changes at the guanine oxidation signal. The oxidation signals of DCB and guanine were respectively measured before/after surface confined interaction process using differantial pulse voltammetry (DPV) in the same voltammetric scale. The experimental parameters, such as DCB concentration and interaction time of DCB with fsDNA were studied. The detection of interaction between DCB and nucleic acids was also studied via electrochemical impedance spectroscopy (EIS) and gel electrophoresis. In addition, the effect of time‐dependent manner from 1 min to 10 min on the interaction of DCB with nucleic acids was explored upon to the changes at guanine signal of fsDNA as well as PCR samples by DPV technique.