Premium
Fabrication of β‐Cyclodextrin/Glycine/Carbon Nanotubes Electrochemical Neurotransmitters Sensor – Application in Ultra‐sensitive Determination of DOPAC in Human Serum
Author(s) -
Galal Ahmed,
Atta Nada F.,
ElAds Ekram H.,
ElGohary Asmaa R. M.
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800046
Subject(s) - detection limit , carbon nanotube , electrochemistry , electrochemical gas sensor , repeatability , selectivity , chemistry , electrode , glycine , cyclodextrin , materials science , analytical chemistry (journal) , chromatography , nanotechnology , organic chemistry , amino acid , biochemistry , catalysis
In this work, an electrochemical sensor was constructed by applying two successive thin layers of glycine‐carbon nanotubes mixture and β‐cyclodextrin (CNTs‐Gly)/CD over glassy carbon electrode surface for some neurotransmitters determination. A host‐guest interaction between CD and neurotransmitters molecules is expected and resulted in enhanced sensitivity, selectivity and stability of sensor response. Other components of the sensor are crucial for the unique electrochemical response. Carbon nanotubes allowed large surface area for glycine distribution that provided hydrogen bonding to CD moieties and contributed to facilitated charge transfer. It was possible to determine 3,4‐dihydroxy phenyl acetic acid (DOPAC) in the linear range of 0.1 μmol L −1 to 80 μmol L −1 with detection limit of 9.40 nmol L −1 , quantification limit of 31.5 nmol L −1 and sensitivity of 4.16 μA/μmol L −1 . The proposed sensor was applied in synthetic cerebrospinal fluids samples using random standard addition method. Also, the proposed sensor was used to determine DOPAC in presence of common interferences and acceptable recovery results were achieved for its analysis in real blood serum. Figures of merit for (CNTs‐Gly)/CD composite in terms of precision, robustness, repeatability and reproducibility were reported.