Premium
Detection of Adrenaline in Blood Plasma as Biomarker for Adrenal Venous Sampling
Author(s) -
Molinnus Denise,
Hardt Gabriel,
Siegert Petra,
Willenberg Holger S.,
Poghossian Arshak,
Keusgen Michael,
Schöning Michael J.
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201800026
Subject(s) - biosensor , amperometry , detection limit , chemistry , venous blood , chromatography , blood sampling , biomarker , biochemistry , endocrinology , electrode , medicine , electrochemistry
An amperometric bi‐enzyme biosensor based on substrate recycling principle for the amplification of the sensor signal has been developed for the detection of adrenaline in blood. Adrenaline can be used as biomarker verifying successful adrenal venous sampling procedure. The adrenaline biosensor has been realized via modification of a galvanic oxygen sensor with a bi‐enzyme membrane combining a genetically modified laccase and a pyrroloquinoline quinone‐dependent glucose dehydrogenase. The measurement conditions such as pH value and temperature were optimized to enhance the sensor performance. A high sensitivity and a low detection limit of about 0.5–1 nM adrenaline have been achieved in phosphate buffer at pH 7.4, relevant for measurements in blood samples. The sensitivity of the biosensor to other catecholamines such as noradrenaline, dopamine and dobutamine has been studied. Finally, the sensor has been successfully applied for the detection of adrenaline in human blood plasma.