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Electrochemical DNA Biosensor Based on Magnetite/Multiwalled Carbon Nanotubes/Chitosan Nanocomposite for Bacillus Cereus Detection of Potential Marker for Gold Prospecting
Author(s) -
Zuo Limei,
Qu Qing,
Li Lei,
Ran Xin,
Gui Jingwei,
Wang Qiang,
Cui Xinhua,
Jiang Changliang
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700801
Subject(s) - detection limit , biosensor , differential pulse voltammetry , nanocomposite , chitosan , dielectric spectroscopy , bacillus cereus , nuclear chemistry , cyclic voltammetry , carbon nanotube , materials science , chemistry , analytical chemistry (journal) , electrochemistry , electrode , nanotechnology , chromatography , organic chemistry , biology , bacteria , genetics
A label‐free DNA biosensor based on magnetite/multiwalled carbon nanotubes/chitosan (Fe 3 O 4 /MWCNTs‐COOH/CS) nanomaterial for detection of Bacillus cereus DNA sequences was fabricated. Negatively charged DNA was electrostatically adsorbed onto materials by protonation of positively charged chitosan under acidic conditions. The electrode surface and hybridization process were carried out by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the biosensor showed a good linear relationship between peak currents difference (ΔI) and logarithm of the target DNA concentration (Log C) ranging from 2.0×10 −13 to 2.0×10 −6 M with a detection limit of 2.0×10 −15 M (signal/noise ratio of 3). The biosensor also revealed an excellent selectivity to three‐base, completely mismatched and completely matched DNA. This is a simple, fast and friendly method with a low detection limit for the detection of Bacillus cereus specific DNA compared with previously reported electrochemical DNA biosensor. Furthermore, the DNA biosensor may lead to the development of a technology for gold prospecting in the wild.