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Multiple Amplified Electrochemical Detection of MicroRNA‐21 Using Hierarchical Flower‐like Gold Nanostructures Combined with Gold‐enriched Hybridization Chain Reaction
Author(s) -
Zhu Dan,
Liu Wei,
Cao Wenfang,
Chao Jie,
Su Shao,
Wang Lianhui,
Fan Chunhai
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700696
Subject(s) - colloidal gold , electrode , detection limit , electrochemistry , materials science , dna–dna hybridization , nanostructure , linear range , substrate (aquarium) , dna , chain reaction , hybridization probe , nanotechnology , nanoparticle , combinatorial chemistry , chemistry , chromatography , photochemistry , biochemistry , biology , ecology
In this work, we present a multiple amplified electrochemical method that can detect target microRNA‐21 (miRNA‐21) with high sensitivity by using hierarchical flower‐like gold nanostructures (HFGNs) and gold‐enriched enzyme‐free amplification. The HFGNs deposited on ITO conducting glass served as the electrode substrate with large specific surface area, which allowed the immobilization of large amounts of capture DNA (DNA‐1). Then, the gold‐enriched hybridization chain reaction (HCR) for signal amplification was attached onto the electrode through target miRNA‐mediated sandwich hybridization. The initial strands (I‐DNA) enriched on gold nanoparticles (AuNPs) provided plenty of sites for the triggering of the HCR reaction after the addition of H1 and H2 strands, which provided exponential increase of binding sites for the loading of electrochemical signal molecules [Ru(NH 3 ) 6 ] 3+ . Therefore, multiple signal amplification could be achieved to reach an ultrasensitive detection of miRNA‐21. A wide linear dynamic range from 1 fM to 1 nM and a detection limit of 0.12 fM (S/N=3) was reached with good selectivity. The electrochemical assay method in this work may hold a great potential for clinical diagnosis of genetic disease in the future.