Premium
An Electrochemical Biosensor with Dual Signal Outputs for Ratiometric Monitoring the Levels of H 2 O 2 and pH in the Microdialysates from a Rat Brain
Author(s) -
Li Shuai,
Tian Yang
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700684
Subject(s) - biosensor , electrochemistry , chemistry , microelectrode , hydrogen peroxide , ischemia , in vivo , myoglobin , electrode , analytical chemistry (journal) , chromatography , biochemistry , medicine , microbiology and biotechnology , biology
The serious damage of ischemia to brain function has attracted extensive attention. As two of vital signal molecules, development of biosensors for monitoring of hydrogen peroxide (H 2 O 2 ) and pH in vivo is significant to understand the roles of H 2 O 2 and pH playing in rat brain followed by ischemia. Herein, a selective and accuracy ratiometric electrochemical biosensor was developed for quantifying H 2 O 2 and pH through both current and potential outputs in a rat brain upon ischemia. Catalase (Cat) was first employed as a specific recognition element for both H 2 O 2 and pH. Meanwhile, an inner reference molecule, ferrocene (Fc), independent of H 2 O 2 and pH, was used as a built‐in correction to improve the accuracy. The reduction peak current of Cat increased with increasing concentration of H 2 O 2 from 1.0 to 230 μM. Meanwhile, the peak potential negatively shifted with increasing pH from 5.91 to 7.81, resulting in determination of H 2 O 2 and pH. Thus, combined with the remarkable analytical performance of the developed biosensor and the desirable biocompatibility of carbon fibre microelectrode (CFME), a direct and reliable approach was established to monitoring H 2 O 2 and pH in the microdialysates collected from rat brain followed by cerebral ischemia.