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Voltammetric Determination of Serine Using L‐Cysteine Modified Platinum Electrode and Brief Exploration from the Theoretical Perspective
Author(s) -
Xue Bingchun,
Fu Yan,
Li Jing,
Liu Erbao
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700682
Subject(s) - detection limit , serine , platinum , adsorption , cysteine , chemistry , electrode , voltammetry , electrochemistry , cyclic voltammetry , analytical chemistry (journal) , inorganic chemistry , chromatography , catalysis , organic chemistry , phosphorylation , biochemistry , enzyme
A new method for the detection of serine was described using L‐cysteine chemically modified electrode, which was prepared by cyclic voltammetry. The factors, such as pH, scan rate, and soaking time for the experimental process were discussed. Under the optimal conditions, the linear range for serine concentration was 3×10 −7 ∼8×10 −5 mol L −1 , the detection limit was 2.34×10 −7 mol L −1 (S/N=3), and the relative standard deviation was 2.14 % (n=10) for 3×10 −7 mol L −1 serine solution. The method was applied to the detection of serine in apple cider vinegar sample with a recovery of 97.5 %–101.9 %. To further discuss the change of the property of platinum surface on atomic level, theoretical calculations were carried out. The change of adsorption energy indicated that the possible interaction of Pt and cysteine was O−C−O bridge adsorption. The analysis about the density of states (DOS) of the system and pseudo band gap showed an increase in the Pt surface activity.