Premium
Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection
Author(s) -
Erdem Arzum,
Congur Gulsah
Publication year - 2018
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700462
Subject(s) - dna , electrochemistry , graphite , electrode , nanoparticle , materials science , biosensor , dna–dna hybridization , selectivity , hybridization probe , oligonucleotide , nanotechnology , nuclear chemistry , chemistry , biochemistry , catalysis , composite material
Abstract Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequence‐selective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP‐PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non‐complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1 : 1) or target: MM (1 : 1).