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Tannic Acid Modified Electrochemical Biosensor for Glucose Sensing Based on Direct Electrochemistry
Author(s) -
Çakıroğlu Bekir,
Özacar Mahmut
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700420
Subject(s) - biosensor , glucose oxidase , graphene , tannic acid , cyclic voltammetry , redox , oxide , nanocomposite , electron transfer , electrochemistry , chemistry , colloidal gold , nanoparticle , materials science , nuclear chemistry , nanotechnology , inorganic chemistry , organic chemistry , electrode
A novel glucose biosensor was constructed through the immobilization of glucose oxidase (GOx) on gold nanoparticles (Au NPs) deposited, and chemically reduced graphene oxide (rGO) nanocomposite. In the synthesis, tannic acid (TA) was used for the reduction of both graphene oxide, and Au 3+ to rGO, and Au NPs, respectively. Also, by harnessing the π‐π interaction between graphene oxide and TA, and protein‐TA interaction, a novel nanocomposite for the fabrication of a third generation biosensor was successfully constructed. Upon the oxidation of TA to quinone, which is easily reducible at the negative potential range, enhanced electron transfer was obtained. The cyclic voltammetry (CV) results demonstrated a pair of well‐defined and quasi‐reversible redox peaks of active site molecule of GOx. The biosensor exhibited a linear response to glucose concentrations varying from 2 to 10 mM with a sensitivity of 18.73 mA mM −1 cm −2 . The fabricated biosensor was used for the determination of glucose in beverages.