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A Novel Enzymatic Glucose Biosensor and Nonenzymatic Hydrogen Peroxide Sensor Based on (3‐Aminopropyl) Triethoxysilane Functionalized Reduced Graphene Oxide
Author(s) -
Guler Muhammet,
Turkoglu Vedat,
Kivanc Mehmet Riza
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700417
Subject(s) - triethoxysilane , graphene , amperometry , glucose oxidase , fourier transform infrared spectroscopy , biosensor , cyclic voltammetry , dielectric spectroscopy , materials science , electrochemical gas sensor , nuclear chemistry , hydrogen peroxide , nafion , electrode , inorganic chemistry , electrochemistry , chemistry , chemical engineering , organic chemistry , nanotechnology , engineering , composite material
In the present study, a novel enzymatic glucose biosensor using glucose oxidase (GOx) immobilized into (3‐aminopropyl) triethoxysilane (APTES) functionalized reduced graphene oxide (rGO‐APTES) and hydrogen peroxide sensor based on rGO‐APTES modified glassy carbon (GC) electrode were fabricated. Nafion (Nf) was used as a protective membrane. For the characterization of the composites, Fourier transform infrared spectroscopy (FTIR), X‐ray powder diffractometer (XRD), and transmission electron microscopy (TEM) were used. The electrochemical properties of the modified electrodes were investigated using electrochemical impedance spectroscopy, cyclic voltammetry, and amperometry. The resulting Nf/rGO‐APTES/GOx/GC and Nf/rGO‐APTES/GC composites showed good electrocatalytical activity toward glucose and H 2 O 2 , respectively. The Nf/rGO‐APTES/GC electrode exhibited a linear range of H 2 O 2 concentration from 0.05 to 15.25 mM with a detection limit (LOD) of 0.017 mM and sensitivity of 124.87 μA mM −1 cm −2 . The Nf/rGO‐APTES/GOx/GC electrode showed a linear range of glucose from 0.02 to 4.340 mM with a LOD of 9 μM and sensitivity of 75.26 μA mM −1 cm −2 . Also, the sensor and biosensor had notable selectivity, repeatability, reproducibility, and storage stability.