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Novel Enzymatic Rhodium Modified Poly(styrene‐ g ‐oleic amide) Film Electrode for Hydrogen Peroxide Detection
Author(s) -
Samet Kilic Muhammet,
Korkut Seyda,
Hazer Baki
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700332
Subject(s) - hydrogen peroxide , rhodium , horseradish peroxidase , detection limit , biosensor , electrode , chemistry , styrene , amide , materials science , catalysis , copolymer , chromatography , organic chemistry , polymer , enzyme , biochemistry
Newly synthesized poly(styrene‐ g ‐oleic amide) was coated onto a rhodium nanoparticle modified glassy carbon (GC) surface for the fabrication of horseradish peroxidase based biosensor used for hydrogen peroxide detection. The rhodium modifed electrode presented ten times higher signal than unmodified electrode even at low elecrtroactive enzyme quantity by enhancing the electron transfer rate at the applied potential of −0.65 V. The biosensor designed by under the optimized rhodium electrodeposition time exhibited a fast response less than 5 s, an excellent operational stability with a relative standard deviation of 0.6 % (n=6), an accuracy of 96 % and a large linear range between 50 μM and 120 mM for hydrogen peroxide. Detection limit and the sensitivity parameters were calculated to be 44 μM and 57 μA mM −1 cm −2 , respectively by preserving its entire initial response up to the 15 days, while only 20 % of its initial response was lost at the end of one month.